Gel Electrophoresis Boot Camp

This practical training is aimed at students and researchers who are applying or planning to use gel electrophoresis in their research. The aim of this training is to equip the participants with practical skills on how to separate proteins using different gel electrophoresis techniques and how to visualize, explore, analyse, assemble, and submit 2D gel data using bioinformatics tools.

• Kshs. 20,000/- (Inclusive of VAT 16%) for East African residents. Non-East African students pay 25% more on all charges.Fee covers tuition, training material, certificate, drinks and meals only.

• Groups with a minimum of 5 people will enjoy a 10% discount and early bird registration (at least 2 weeks before the start) will be offered a 5% discount

Terms and conditions of Payments:

• Fees are payable strictly in advance, (4 working days before the starting date). Cancellations made within 5 working days of a course will incur a 20% cancellation fee. We regret that no cancellations will be accepted within 2 working days of a course, but substitutions may be made.No
• There will be a 10% administrative charge for cancellations received in writing up to 20 working days before the start of the course. No refunds will be made for cancellations received within 20 working days of the course start date or for the inability to attend the course for whatever reasons. KIBs is not liable for non-attendance due to travel disruptions, health problems or any other reason that might lead to a delegate not being able to attend the course. Substitutions may, of course, be made at any time, providing you inform us in writing.
• Fee is payable by bankers cheque or cash deposit to: Fee is payable by bankers cheque or MPESA to: Bioinformatics Institute of Kenya. A/C No. 01192845321700, Cooperative Bank, Co-op House Branch, Nairobi, Kenya. SWIFT Code KCOOKENAXXX. Branch Code: XXX. MPESA PAY BILL: Business number: 400222. Account number: 1679821#2DGE. PAYPAL: [email protected].

To register, please download the booking form fill it and send it to us through: Email: [email protected].

1. Introduction to gel electrophoresis

• Separation theory and optimization
• SDS-PAGE (Laemmli) Buffer System
• Native PAGE
• PH and pI
• Choosing Native PAGE Systems

2. Identification of the gel electrophoresis components:

• Spacer plate
• Short plate
• Casting frame
• Gel cassette assembly
• Combs
• Buffer dam
• Electrode assembly
• Clamping frame
• Inner chamber
• Mini-tank and lid

3. Reagent preparation and stock solutions

• Volumes required per gel
• SDS-PAGE (Laemmli) Buffer System
• Discontinuous Native PAGE (Ornstein-Davis)
• Continuous Native PAGE (Ornstein-Davis)

4. Set up and basic operation of the gel electrophoresis system

• Gel cassette sandwich preparation (hand cast and ready cast gels)
• Glass cassette and casting stand assembly
• Gel casting for continuous and discontinuous polyacrylamide gels
• Ready gel precast gels
• Module assembly and sample loading
• Gel electrophoresis
• Mini tank assembly
• Power conditions
• Gel removal

5. Role of bioinformatics in 2D-Gel Electrophoresis

• Segmentation-oriented methods
• Surface-oriented methods
• Visualization, exploration, analysis, assembly, and submission of 2D gel data using SWISS-2DPAGE, World-2DPAGE Constellation,World-2DPAGE Repository, Melanie, Make2D-DB II,andMiapeGelDB

6. Maintenance of the gel electrophoresis system

7. Exercises

8. Award of certificates