Serum Protein Electrophoresis Test (SPEP)

Synonyms

None

Purpose

Serum Protein Electrophoresis Test separates specific proteins in the blood using electric charge. Used in detection of monoclonal gammopathies.

Patient Preparation

No special preparation needed.

Samples

2ml with a minimum volume of 1ml of serum collected in a red top tube or gel barrier tube. Serum should be separated within 45 minutes of sample collection. Sample should be refrigerated at 2-8C.

Reference Range

0-6months:4.6-7.2g/dl,7m to 1year:5.7-8.2g/dl,.1 year:6.0-8.5g/dl.

Results Turnaround

3-4 days after receipt of samples.

Diseases / Diagnostic indicators

Decreased total protein with normal pattern indicates dietary deficiency or heodilution while elevated protein with a normal pattern is secondary to dehydration.